Ling-Qin Song1,
Hua-Feng Kang1 
,
Di Liu1,
Zhi-Jun Dai1,
Jian-Jun He2,
Bao-Feng Wang1,
Yang Zhao1,
Xi-Jing Wang1,
Wen-Tao Xi1
For correspondence:- Hua-Feng Kang Email: kanghuafeng23@gmail.com Tel:+862987679513
Received: 14 November 2014 Accepted: 21 July 2015 Published: 29 September 2015
Citation: Song L, Kang H, Liu D, Dai Z, He J, Wang B, et al. Dimedone derivative {2-[(4-hydroxy-phenylamino)-methylene]-5,5-dimethyl-cyclohexane-1,3-dione} plays an important role in breast cancer treatment. Trop J Pharm Res 2015; 14(9):1719-1722 doi: 10.4314/tjpr.v14i9.25
© 2015 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To investigate the effect of 2-[(4-hydroxy-phenylamino)-methylene]-5,5-dimethyl-cyclohex (HPDH) on mammary carcinogenesis induced by 7,12-dimethylbenz(a)anthracene in female Sprague Dawley rats.
Methods: Among three groups of rats (50 each) used in the study, the control group was fed standard diet alone, ibuprofen group received standard diet containing 1200 mg/kg ibuprofen while HPDH group was administered standard diet containing 1200 mg/kg HPDH. The treatment was for 10 days for all the groups. All the animals received 20 mg of DMBA intragastrically by gavage. Clinical parameters for the rats were recorded weekly. Micrometer caliper was used to measure the diameter of all the tumors at the end of the experiment and tumor volume calculated. Histological evaluation was performed using hematoxylin and eosin (H&E) staining. High performance liquid chromatography (HPLC) was used to determine the level of HPDH and ibuprofen in the serum of the animals.
Results:The data revealed a significant decrease in the number of rats with mammary tumor, number of tumors/rat and tumor volume by 54, 72 and 75 %, respectively, in HPDH group compared to control group. The ibuprofen-treated rats also showed significant decrease in the number of rats with tumor, number of tumors/rat and tumor volume by 43, 55, and 59 %, respectively. Treatment of rats with HPDH increased the latency period of tumor induction significantly (p < 0.005). Median detection period (50 % of tumors) was 92, 83 and 56 days, respectively, in HPDH, ibuprofen and control groups, respectively, after DMBA induction.
Conclusion: These results demonstrate that HPDH possesses strong chemopreventive activity against mammary carcinogenesis.
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